• Corpus ID: 212542253

Optimization of Polyhydroxyalkanoates (PHA) Production From Liquid Bean Curd Waste by Alcaligenes Latus Bacteria

  title={Optimization of Polyhydroxyalkanoates (PHA) Production From Liquid Bean Curd Waste by Alcaligenes Latus Bacteria},
  author={Sri Kumalaningsih and Nur Hidayat and Nurul Aini},
Study to obtain the optimum condition for the production of PHAs by Alcaligenes latus was carried using liquid bean curd waste as low cost carbon source. A Response surface method with two factors i.e. the initial sucrose concentration (15 g/L, 20 g/L, and 25 g/L) and time of incubation (48 hours, 60 hours, and 72 hours) was employed. The yield was taken after the inoculation A. latus on to the liquid bean curd waste. The results obtained from the study are computed with Design expert 7.1.3… 

Figures and Tables from this paper

Poly-3-hydroxyalkanoates production potential of Bacillus cereus C113 isolated from cassava dumpsite using some carbon sources
Poly-3-hydroxyalkanoates (PHAs) are biopolymers accumulated as intracellular energy reserves by bacteria under nutrient limiting growth conditions and suitable for plastic production. We report
A higher in value biopolymer product of polyhydroxyalkanoates (PHAs) synthesized by Alcaligenes latus in batch/repeated batch fermentation processes of sugar cane juice
The physicochemical properties of nuclear magnetic resonance (NMR) and a fourier transform infrared (FTIR) spectrogram were characterized and revealed to be in full agreement with the standard polymer of polyhydroxybutyrate (PHB).
Production of biodegradable plastic by polyhydroxybutyrate (PHB) accumulating bacteria using low cost agricultural waste material
The isolated Bacillus sp can be used for feasible production of PHB using agro-residues especially sugarcane bagasse which can reduce the production cost in addition to reducing the disposal problem of these substrates.
The bacterium isolated from the cloth used to smear oil on pan cake pan seems to be a potential candidate for the biogenic synthesis of PHB which can find application as drug carrier.
Food waste conversion to microbial polyhydroxyalkanoates
This article's main focus is to examine the state of the art of converting food‐derived waste into carbon substrates for microbial metabolism and subsequent conversion into PHAs.
Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review
Optimization strategies need to be logically integrated for the successful commercialization of microbial PHAs, and experimental design-based bioprocess, use of metabolic engineering tools, and optimal product recovery techniques are invaluable in this dimension.
Overview on Polyhydroxyalkanoates: A Promising Biopol
Plastic pollution is creating the significant environmental and economic burdens since they consume the natural fuels (energy) and other natural resources. Beside this, they have long shelf time,


Batch Production of Polyhydroxybutyrate (PHB) by Ralstonia Eutropha and Alcaligenes Latus Using Bioreactor Different Culture Strategies
Ralstonia eutropha ATCC 17697 and Alcaligenes l atus ATCC 29712 were t ested f or the i ntracellular bioplastic pol y-â-hydroxybutyric a cid ( PHB) production us ing bi oreactor as batch, two-stage batch and Fed batch cultures.
Poly(3-Hydroxybutyrate) Production with High Productivity and High Polymer Content by a Fed-Batch Culture of Alcaligenes latus under Nitrogen Limitation.
  • F. WangS. Lee
  • Engineering, Biology
    Applied and environmental microbiology
  • 1997
In flask and batch cultures, the PHB synthesis rate could be increased considerably by applying nitrogen limitation, which was considerably higher than that typically obtainable under nitrogen-sufficient conditions.
Increased PHB productivity by high‐cell‐density fed‐batch culture of Alcaligenes latus, a growth‐associated PHB producer
Alcaligenes latus, a growth‐associated PHB producer, was cultivated by a pH‐stat modal fed‐batch culture technique to attain high PHB productivity. Both sucrose solution and inorganic medium were fed
An extracellular poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis.
Analysis of hydrolytic products of poly(3-hydroxybutyrate), several oligomeric esters of D(--)-3-Hydroxybutyric acid, and the methyl ester of the trimeric ester indicated that the enzyme hydrolyzed these substrates from the free hydroxyl terminus, releasing D(.--)-3,hydroxy butyrate dimer units one at a time.
Quantitative Determination of the Biodegradable Polymer Poly(β-hydroxybutyrate) in a Recombinant Escherichia coli Strain by Use of Mid-Infrared Spectroscopy and Multivariative Statistics
F Fourier transform infrared spectroscopy in combination with the partial least squares (PLS) multivariative statistical technique was used for quantitative analysis of the poly(β-hydroxybutyrate) (PHB) contents of bacterial cells, demonstrating the potential of FTIR spectroscopic as an alternative to the conventional methods used for analysis of PHB in bacterial cells.
Microbial production of polyhydroxy alkanotes (PHA) from Alcaligens spp. and Pseudomonas oleovorans using different carbon sources
Biodegrability studies showed that, the PHA produced is degradable by a number of soil microbes making it an ideal environmentally friendly material for regular human use.
Polyesters from microorganisms.
The bacterial production and properties of poly(3-hydroxyalkanoates) and poly(beta-malate) are described with emphasis on the former.
Poly (ß-hydroxybutiric acid) thermoplastic production by Alcaligenes latus: Behavior of fed-batch cultures
  • Bioprocess Engineering V
  • 2000