Optimization of Parameters in a DNA Sequenator Using Fluorescence Detection

Abstract

We have developed an automated DNA sequenator using a real-time fluorescence detection method, and achieved excellent sensitivity and rapid analysis by optimizing operating conditions. The sequenator uses unicolor labeling and four-track electrophoresis. Fluoresceine isothiocyanate(FITC)-labeled primers are used to produce labeled DNA families. The… (More)
DOI: 10.1038/nbt0788-816

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