Optimization of PCR conditions to amplify mitochondrial COI gene fragments of wildlife species in Kenya

@inproceedings{Kimwele2012OptimizationOP,
  title={Optimization of PCR conditions to amplify mitochondrial COI gene fragments of wildlife species in Kenya},
  author={Charles N Kimwele and Erastus Gatebe and Zipporah Osiemo and Awt Muigai and Mary Nyiva Mwaniki and Julius Kinuthia and E Song'ore},
  year={2012}
}
PCR has been extensively used for amplification of DNA sequences. We conducted a study to obtain the best amplification conditions for cytochrome c oxidase I (COr) gene fragments of some of the targeted wildlife species in Kenya; butTalo,common zebra, grant's gazelle, warthog and common eland and domestic samples purchased from the market as 'beef', 'goat'or 'mutton'. DNA from five wildlife species and one hundred of domestic samples were extracted for PCR amplification. Various trials and… CONTINUE READING