Optimization of Media and Feeding Strategy for Improved Capsular Polysaccharide Yield for Neisseria meningitidis W-135

Abstract

--------------------------------------------------------ABSTRACT----------------------------------------------------------Meningococcal serogroup W-135 (MenW-135) has turned out to be the reason for recent outbreak of meningitis in the sub-Saharan regions of Africa including Cameroon, Chad, Gambia, Burkina Faso, and other countries. The capsular polysaccharide (PS) of Neisseria meningitidis serogroups A, C, X, Y, and W-135 are excellent vaccine targets against meningitis caused by this bacterium. This study promotes a novel feed solution including amino acids, vitamins, and glucose along with an optimal fermentation medium; and feeding strategy to cultivate high yield polysaccharide at harvest level. The study is based on the hypothesis that glucose is one of the major growth determining factors with the combinations of nitrogen sources. In this study, fed-batch cultivation was performed in a 2L fermenter, maintaining the following conditions: (i) Temperature = 37oC (ii) pH = 7.0 (iii) Agitation frequency in the range 150 rotations per minute (rpm) to 500 rpm, and (iv) Dissolved oxygen (DO) 20%-25%. The bacterial growth and polysaccharide production were found to be enhanced when the feed flow was increased periodically from late lag phase to early decline phase. The polysaccharide production was found to be maximum in the early decline phase of the bacterial growth. The said method produced polysaccharide of about 1000mg/L, which is twice the reported method. There is limited research information available on fed-batch cultivation and feeding strategy for growing Neisseria meningitidis W-135. The major findings of this study can provide significant contribution in the field of capsular polysaccharide production from which MenW polysaccharide vaccines are manufactured.

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Cite this paper

@inproceedings{Kambrath2017OptimizationOM, title={Optimization of Media and Feeding Strategy for Improved Capsular Polysaccharide Yield for Neisseria meningitidis W-135}, author={Sujitha Valiya Kambrath and Srinivas Reddy and Sambhaji S Pisal and Rajeev M. Dhere}, year={2017} }