Optimization of Intracellular Product Release from Neisseria denitrificans Using Microfluidizer

  title={Optimization of Intracellular Product Release from Neisseria denitrificans Using Microfluidizer},
  author={Robert J. Stupak and Nerijus Makauskas and Kostas Radzevi{\vc}ius and Zenonas Valan{\vc}ius},
  journal={Preparative Biochemistry and Biotechnology},
  pages={667 - 683}
Disruption of Neisseria denitrificans cells by microfluidizer was optimized using a factorial experiments design. The pH, pretreatment time, cell concentration, NaCl, ethylenediamine tetraacetic acid (EDTA) and Triton X-100 concentrations showed significant impact on disruption process and the process was optimized using central composite design and response surface methodology (RSM). Investigation revealed optimum conditions: 90 min pretreatment at pH 9.0 containing 110 g L−1 cells (dry cell… 

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