Optimization of Intracellular Product Release from Neisseria denitrificans Using Microfluidizer

@article{Stupak2015OptimizationOI,
  title={Optimization of Intracellular Product Release from Neisseria denitrificans Using Microfluidizer},
  author={Robert J. Stupak and Nerijus Makauskas and Kostas Radzevi{\vc}ius and Zenonas Valan{\vc}ius},
  journal={Preparative Biochemistry and Biotechnology},
  year={2015},
  volume={45},
  pages={667 - 683}
}
Disruption of Neisseria denitrificans cells by microfluidizer was optimized using a factorial experiments design. The pH, pretreatment time, cell concentration, NaCl, ethylenediamine tetraacetic acid (EDTA) and Triton X-100 concentrations showed significant impact on disruption process and the process was optimized using central composite design and response surface methodology (RSM). Investigation revealed optimum conditions: 90 min pretreatment at pH 9.0 containing 110 g L−1 cells (dry cell… 

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References

SHOWING 1-10 OF 31 REFERENCES

Optimization of acidic extraction of astaxanthin from Phaffia rhodozyma

This acidic method has advantages such as high extraction efficiency, low chemical toxicity and no special requirement of instruments, therefore, it might be a more feasible and practical method for industrial practice.

EVALUATION OF DISRUPTION METHODS FOR RELEASING INTRACELLULAR PRODUCT FROM COCCUS AND BACILLUS TYPE BACTERIAL CELLS

US and traditional construction HPH were determined to be cell-geometry-dependent methods, less effectively disrupting the Nocardia corallina bacilus in comparison with the Neisseria denitrificans coccus, while Microfluidizer, opposedsteam HPH, was found to be a cell-type-independent method with the lowest energy consumption and highest disruption rates among the analyzed methods.

Enhanced disruption of Candida utilis using enzymatic pretreatment and high‐pressure homogenization

A modified model was developed to predict the fraction disrupted by the enzymatic pretreatment‐mechanical homogenization two‐stage process, and predicted disruptions agreed favorably with experimental observations (maximum deviation of 20%) over a wide range of operating conditions.

Isolation of biologically active nanomaterial (inclusion bodies) from bacterial cells

Homogenization is the most appropriate method for the isolation of active nanoparticles from bacterial cells, and the surface of IBs appears more porous when compared to the other two methods.

Isolation of cell-free bacterial inclusion bodies

A convenient procedure is established for the recovery of bacterial inclusion bodies with undetectable levels of viable cell contamination, below 10-1 cfu/ml, keeping the particulate organization of these aggregates regarding size and protein folding features.