Optimization of Immobilization Conditions of Candida antarctica Lipase Based on Response Surface Methodology

@inproceedings{Liu2010OptimizationOI,
  title={Optimization of Immobilization Conditions of Candida antarctica Lipase Based on Response Surface Methodology},
  author={Junhong Liu and Yuanyuan Zhang and Yuanxian Xia and Feng Su},
  year={2010}
}
The conditions, including mass ratio of PEG4000 to lipase, pH, and mass ratio of diatomites to lipase, for immobilization of Candida antarctica lipase with PEG non-covalent modification were optimized by means of the response surface methodology (RSM). The immobilized lipase specific activity in the reaction of transesterification was selected as the response value. A mathematical model was developed to investigate the influences of various immobilization parameters and to predict the optimum… CONTINUE READING

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