Optimization of Fermentation Conditions of Genetically Engineered Pichia pastoris Expressing Recombinant Human-source Collagen

@inproceedings{Shulin2011OptimizationOF,
  title={Optimization of Fermentation Conditions of Genetically Engineered Pichia pastoris Expressing Recombinant Human-source Collagen},
  author={Yang Shu-lin},
  year={2011}
}
Response surface methodology(RSM)is used to optimize the fermentation conditions of genetically engineered Pichia pastoris(GS115/pPIC9KG6)expressing highly hydrophilic recombinant human-source collagen.Box-Behnken design(BBD)is introduced to estimate the impact of initial inducement pH,temperature and addition amount of methanol.An experiment is done by setting different culture conditions according to the scheme designed through the DX7Trial software,and the correlational parameters are… CONTINUE READING

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