Optimization of DNA extraction from fresh leaf tissues of Melanoxylon brauna (Fabaceae).

@article{Borges2012OptimizationOD,
  title={Optimization of DNA extraction from fresh leaf tissues of Melanoxylon brauna (Fabaceae).},
  author={D. B. Borges and M. B. Amorim and A. M. Waldschmidt and E. Mariano-Neto and C. V. Vivas and D. G. Pereira},
  journal={Genetics and molecular research : GMR},
  year={2012},
  volume={11 2},
  pages={
          1586-91
        }
}
Melanoxylon brauna (Fabaceae - Caesalpinioideae) is an endemic and valuable hardwood tree species in the Brazilian Atlantic rainforest; it is comparable to African ebony wood. We tested three protocols of DNA extraction based on the citrimonium bromide (CTAB) method and evaluated the quantity, purity and integrity of the DNA. We also determined whether these procedures interfere with PCR amplification in order to develop a protocol for M. brauna. We found that the quality and integrity of DNA… Expand
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