Optimization for speed and sensitivity in capillary high performance liquid chromatography. The importance of column diameter in online monitoring of serotonin by microdialysis.

@article{Zhang2012OptimizationFS,
  title={Optimization for speed and sensitivity in capillary high performance liquid chromatography. The importance of column diameter in online monitoring of serotonin by microdialysis.},
  author={Jing Zhang and Yansheng Liu and Andrea S. Jaquins-Gerstl and Zhan Shu and Adrian C Michael and Stephen G Weber},
  journal={Journal of chromatography. A},
  year={2012},
  volume={1251},
  pages={54-62}
}
The speed of a separation defines the best time resolution possible in online measurements using chromatography. The desired time resolution multiplied by the flow rate of the stream of analyte being sampled defines the maximum volume of sample per injection. The best concentration sensitivity in chromatography is obtained by injecting the largest volume of sample that is consistent with achieving a satisfactory separation, and thus measurement accuracy. Taking these facts together, it is easy… CONTINUE READING