Optimization and evaluation of metabolite extraction protocols for untargeted metabolic profiling of liver samples by UPLC-MS.

@article{Masson2010OptimizationAE,
  title={Optimization and evaluation of metabolite extraction protocols for untargeted metabolic profiling of liver samples by UPLC-MS.},
  author={Perrine Anne Sophie Masson and Alexessander Couto Alves and Timothy M. D. Ebbels and Jeremy K. Nicholson and Elizabeth J. Want},
  journal={Analytical chemistry},
  year={2010},
  volume={82 18},
  pages={
          7779-86
        }
}
A series of six protocols were evaluated for UPLC-MS based untargeted metabolic profiling of liver extracts in terms of reproducibility and number of metabolite features obtained. These protocols, designed to extract both polar and nonpolar metabolites, were based on (i) a two stage extraction approach or (ii) a simultaneous extraction in a biphasic mixture, employing different volumes and combinations of extraction and resuspension solvents. A multivariate statistical strategy was developed to… CONTINUE READING
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