Optimization and comparison of knockdown efficacy between polymerase II expressed shRNA and artificial miRNA targeting luciferase and Apolipoprotein B100

@inproceedings{Maczuga2012OptimizationAC,
  title={Optimization and comparison of knockdown efficacy between polymerase II expressed shRNA and artificial miRNA targeting luciferase and Apolipoprotein B100},
  author={Piotr Maczuga and Annemart Koornneef and Florie Borel and Harald Petry and Sander J. van Deventer and Tita Ritsema and Pavlina D. Konstantinova},
  booktitle={BMC biotechnology},
  year={2012}
}
BACKGROUND Controlling and limiting the expression of short hairpin RNA (shRNA) by using constitutive or tissue-specific polymerase II (pol II) expression can be a promising strategy to avoid RNAi toxicity. However, to date detailed studies on requirements for effective pol II shRNA expression and processing are not available. We investigated the optimal structural configuration of shRNA molecules, namely: hairpin location, stem length and termination signal required for effective pol II… CONTINUE READING

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