Optimization and characterization of anionic lipoplexes for gene delivery.

  title={Optimization and characterization of anionic lipoplexes for gene delivery.},
  author={Charudharshini Srinivasan and Diane J. Burgess},
  journal={Journal of controlled release : official journal of the Controlled Release Society},
  volume={136 1},
Anionic lipoplexes, comprising divalent cations, DNA and anionic liposomes, were optimized for high transfection efficiency and low cytotoxicity. Different molar ratios of anionic to zwitterionic lipid, 1:9 to 1:1 (1,2-dioleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (DOPG): 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), various cations (Ca2+, Mg2+ and Na+) and different anionic lipid/DNA ratios were investigated. The optimized formulation was composed of: anionic/zwitterionic lipid… CONTINUE READING
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