Optimization and Applications of CDAP Labeling for the Assignment of Cysteines

  title={Optimization and Applications of CDAP Labeling for the Assignment of Cysteines},
  author={Gary D. Pipes and Andrew A. Kosky and Jeffrey S. Abel and Yonghui Zhang and Michael J. Treuheit and Gerd R. Kleemann},
  journal={Pharmaceutical Research},
The aim of the study is to provide a methodology for assigning unpaired cysteine residues in proteins formulated in a variety of different conditions to identify structural heterogeneity as a potential cause for protein degradation. 1-Cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP) was employed for cyanylating free cysteines in proteins and peptides. Subsequent basic cleavage of the peptide bond at the N-terminal side of the cyanylated cysteines provided direct information about their… CONTINUE READING


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