Optimisation of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9:single-guide RNA (sgRNA) delivery system in a goat model.

@article{Huang2019OptimisationOT,
  title={Optimisation of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9:single-guide RNA (sgRNA) delivery system in a goat model.},
  author={Yu Huang and Yige Ding and Yao Liu and Shiwei Zhou and Qiang Ding and Hailong Yan and Baohua Ma and Xiaoe Zhao and Xiaolong Wang and Yulin Chen},
  journal={Reproduction, fertility, and development},
  year={2019}
}
The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system is an efficient method for the production of gene-edited animals. We have successfully generated gene-modified goats and sheep via zygote injection of Cas9 mRNA and single-guide RNA (sgRNA) mixtures. However, the delivery system for microinjection largely refers to methods established for mice; optimised injection conditions are urgently required for the generation of large animals. Here, we designed a study to… 
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Highly efficient generation of sheep with a defined FecBB mutation via adenine base editing
TLDR
The validation of the first gene-edited sheep generated by Adenine base editors is reported and its potential to improve economically important traits in livestock is highlighted.
Redesigning small ruminant genomes with CRISPR toolkit: Overview and perspectives.

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