Optimisation of siRNA-mediated RhoA silencing in neuronal cultures.

  title={Optimisation of siRNA-mediated RhoA silencing in neuronal cultures.},
  author={Ellen L Suggate and Zubair Ahmed and Martin L Read and Kimberley Eaton-Charnock and Michael R. Douglas and A Gonzalez and Martin Berry and Ann Logan},
  journal={Molecular and cellular neurosciences},
  volume={40 4},
In investigating the consequences of gene silencing in axon growth disinhibition strategies in cultured retinal ganglion cells (RGC), we conducted experiments designed to silence RhoA signalling in PC12 and primary adult rat retinal cell cultures (containing RGC) by siRNA-mediated RhoA mRNA knockdown. We demonstrate wide differences in the levels of RhoA mRNA knockdown, dose-dependent cell toxicity, and induction of endogenous inflammatory cytokine and interferon responses to siRNA therapy… CONTINUE READING


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