Optimisation of in ovo electroporation of the chick neural tube.

Abstract

Chick in ovo neural tube electroporation has become a widely used method for assaying gene function during embryonic development. Since its first description, many variants of this technique have been described, with varying values for specific parameters such as electrode type and spacing, voltage, pulse duration and plasmid DNA concentration. Here we… (More)
DOI: 10.1016/j.jneumeth.2011.08.012

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