Optimisation of immobilisation conditions for chick pea β-galactosidase (CpGAL) to alkylamine glass using response surface methodology and its applications in lactose hydrolysis.

@article{Kishore2012OptimisationOI,
  title={Optimisation of immobilisation conditions for chick pea β-galactosidase (CpGAL) to alkylamine glass using response surface methodology and its applications in lactose hydrolysis.},
  author={Devesh Kishore and Arvind Mohan Kayastha},
  journal={Food chemistry},
  year={2012},
  volume={134 3},
  pages={1650-7}
}
Response surface methodology was advantageously used to optimally immobilise a β-galactosidase from chick pea onto alkylamine glass using Box-Behnken experimental design, resulting in an overall 91% immobilisation efficiency. Analysis of variance was performed to determine the adequacy and significance of the quadratic model. Immobilised enzyme showed a… CONTINUE READING