Optimisation of culture conditions to develop an in vitro pulmonary permeability model.

Abstract

An in vitro model to study pulmonary translocation was created, using the human cell line Calu-3 and primary rat type II pneumocytes. Cells were seeded on permeable membranes with a 0.4 microm or 3 microm pore size, utilizing different culture conditions such as medium formulation and cell density. The integrity of the cell monolayer was verified by… (More)

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@article{Geys2007OptimisationOC, title={Optimisation of culture conditions to develop an in vitro pulmonary permeability model.}, author={Jorina Geys and Bert Nemery and P. H. M. Hoet}, journal={Toxicology in vitro : an international journal published in association with BIBRA}, year={2007}, volume={21 7}, pages={1215-9} }