Optimisation of an extraction method for the determination of prostaglandin E2 in plasma using experimental design and liquid chromatography tandem mass spectrometry.

@article{Arajo2006OptimisationOA,
  title={Optimisation of an extraction method for the determination of prostaglandin E2 in plasma using experimental design and liquid chromatography tandem mass spectrometry.},
  author={Pedro Ara{\'u}jo and Livar Fr\oyland},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2006},
  volume={830 2},
  pages={212-7}
}
A new extraction method has been developed for the extraction of prostaglandin E(2) (PGE(2)) from human plasma of patients suffering chronic inflammatory disorders. The extraction solvents were optimised systematically and simultaneously by using a central composite design. The optimised method involves precipitation of the protein fraction, centrifugation, evaporation and dissolution of the supernatant in the mobile phase, screening to confirm the presence of the analyte, and quantification of… CONTINUE READING
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