OptimalConditionsandComparisonof LactateDehydrogenase Catalysisof the Lactate-to-Pyruvateand Pyruvate-to-Lactate


We report optimal conditions for assaying highly purified human lactate dehydrogenase isoenzymes with the lactate-to-pyruvate and pyruvate-to-lactate reactions, as they apply to human serum. Interconversion of results between reactions is not practicable. Measurements of lactate dehydrogenase in either reaction direction at 25, 30, or 37 #{176}C can be… (More)

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