Optimal selection of 2D reversed-phase-reversed-phase HPLC separation techniques in bottom-up proteomics.

@article{McQueen2012OptimalSO,
  title={Optimal selection of 2D reversed-phase-reversed-phase HPLC separation techniques in bottom-up proteomics.},
  author={Peter D McQueen and Oleg V Krokhin},
  journal={Expert review of proteomics},
  year={2012},
  volume={9 2},
  pages={125-8}
}
Recent developments in bottom-up proteomics have supplanted the use of gel-based approaches in favor of multidimensional chromatographic separations of peptide mixtures followed by mass spectrometry analysis. This trend is driven by the desire to eliminate labor-intensive in-gel digestion procedures and increase proteome coverage through better recovery of proteolytic fragments. Introduction of reversed-phase-reversed-phase 2D separation techniques is one of the major improvements that have… CONTINUE READING