Optimal nitrogen supply as a key to increased and sustained production of a monoclonal full-size antibody in BY-2 suspension culture.

@article{Holland2010OptimalNS,
  title={Optimal nitrogen supply as a key to increased and sustained production of a monoclonal full-size antibody in BY-2 suspension culture.},
  author={Tanja Holland and Markus Sack and Thomas Rademacher and Kathrin Schmale and Friedrich Altmann and Johannes Stadlmann and Rainer Fischer and Stephan Hellwig},
  journal={Biotechnology and bioengineering},
  year={2010},
  volume={107 2},
  pages={278-89}
}
Plant cell cultures have been used as expression hosts for recombinant proteins for over two decades. The quality of plant cell culture-produced proteins such as full-size monoclonal antibodies has been shown to be excellent in terms of protein folding and binding activity, but the productivity and yield fell short of what was achieved using mammalian cell culture, in which the key to gram-per-liter expression levels was strain selection and medium/process optimization. We carried out an… CONTINUE READING

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