Optimal growth conditions for the determination of the antifungal susceptibility of three species of dermatophytes with the use of a microdilution method.

@article{Norris1999OptimalGC,
  title={Optimal growth conditions for the determination of the antifungal susceptibility of three species of dermatophytes with the use of a microdilution method.},
  author={Henry A. Norris and B E Elewski and Mahmoud Afif Ghannoum},
  journal={Journal of the American Academy of Dermatology},
  year={1999},
  volume={40 6 Pt 2},
  pages={S9-13}
}
As a prerequisite to standardization of dermatophyte susceptibility testing, conditions that support optimal growth of different dermatophyte species must be established. Eighteen isolates of Trichophyton spp. (T rubrum, T mentagrophytes, T tonsurans) were grown in 4 different media: RPMI 1640 with L-glutamine, without sodium bicarbonate and buffered at pH = 7.0; antibiotic medium #3 (Penassay); yeast nitrogen base with 0.5% dextrose buffered at pH = 7.0; and Sabouraud dextrose broth… CONTINUE READING

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