Optimal degree of protonation for 1H detection of aliphatic sites in randomly deuterated proteins as a function of the MAS frequency

@article{Asami2012OptimalDO,
  title={Optimal degree of protonation for 1H detection of aliphatic sites in randomly deuterated proteins as a function of the MAS frequency},
  author={Sam Asami and Kathrin Sz{\'e}kely and Paul Schanda and Beat H Meier and Bernd Reif},
  journal={Journal of Biomolecular NMR},
  year={2012},
  volume={54},
  pages={155-168}
}
The 1H dipolar network, which is the major obstacle for applying proton detection in the solid-state, can be reduced by deuteration, employing the RAP (Reduced Adjoining Protonation) labeling scheme, which yields random protonation at non-exchangeable sites. We present here a systematic study on the optimal degree of random sidechain protonation in RAP samples as a function of the MAS (magic angle spinning) frequency. In particular, we compare 1H sensitivity and linewidth of a microcrystalline… CONTINUE READING
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