Optimal conditions for the use of sulphydryl compounds in dissociating red cell antibodies.

@article{Freedman1976OptimalCF,
  title={Optimal conditions for the use of sulphydryl compounds in dissociating red cell antibodies.},
  author={John T. Freedman and C A Masters and M Newlands and Patrick L Mollison},
  journal={Vox sanguinis},
  year={1976},
  volume={30 3},
  pages={231-9}
}
Optimal conditions for the use of 2-mercaptoethanol (2-ME) and dithiothreitol (DTT) in the inactivation of IgM red cell antibodies were investigated. 0.2 M 2-ME was better than 0.01 M DTT; higher concentrations of these 2 substances could not conveniently be used because of gel formation. Incubation at 37 degrees C led to far more rapid inactivation than incubation at 22 degrees C; with 2-ME a period of 15 min at 37 degrees C was sufficient except with very potent antibodies, which were not… CONTINUE READING