Optimal conditions for simultaneous purification of mononuclear and polymorphonuclear leucocytes from human blood by the Hypaque-Ficoll method.

@article{Ferrante1980OptimalCF,
  title={Optimal conditions for simultaneous purification of mononuclear and polymorphonuclear leucocytes from human blood by the Hypaque-Ficoll method.},
  author={Antonio Ferrante and Yeong H Thong},
  journal={Journal of immunological methods},
  year={1980},
  volume={36 2},
  pages={109-17}
}
Data on the optimal conditions for the simultaneous purification of mononuclear (MN) and polymorphonuclear (PMN) leucocytes from human blood on Hypaque-Ficoll medium of one density is presented. The efficiency of separation of MN and PMN leucocytes by the Hypaque-Ficoll method was dependent on both the Ficoll concentration and the density of the medium. Best results were obtained with a medium consisting of a Ficoll concentration of 8.2% and a density of 1.114 g/ml. The separation procedure was… CONTINUE READING

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