Optimal concentrations of N-decanoyl-N-methylglucamine and sodium dodecyl sulfate allow the extraction and analysis of membrane proteins.

@article{Chuang2011OptimalCO,
  title={Optimal concentrations of N-decanoyl-N-methylglucamine and sodium dodecyl sulfate allow the extraction and analysis of membrane proteins.},
  author={Jen-Hua Chuang and Yu-Jing Kao and Neil Bertrand Ruderman and Li-Chu Tung and Yenshou Lin},
  journal={Analytical biochemistry},
  year={2011},
  volume={418 2},
  pages={298-300}
}
We studied the extraction and analysis of integral membrane proteins possessing hydrophobic and hydrophilic domains and found that a nonionic detergent called MEGA-10, used in lysis buffers, had a superior extraction effect compared to most conventional detergents. A sodium dodecyl sulfate (SDS) concentration of >0.4% (w/v) in the sample buffer was crucial… CONTINUE READING