Optimal DNA sequence recognition by the Ultrabithorax homeodomain of Drosophila.

@article{Ekker1991OptimalDS,
  title={Optimal DNA sequence recognition by the Ultrabithorax homeodomain of Drosophila.},
  author={Stephen C. Ekker and Keith E. Young and Doris P von Kessler and Philip A Beachy},
  journal={The EMBO journal},
  year={1991},
  volume={10 5},
  pages={1179-86}
}
The 61 amino acid homeodomain is conserved among members of a family of eukaryotic DNA-binding proteins that play regulatory roles in transcription and in development. We have refined a rapid method for determining optimal DNA binding sites and have applied it to a 72 amino acid peptide containing the homeodomain of the Ultrabithorax (Ubx) homeotic gene of Drosophila. The site (5'-TTAATGG-3') is tightly bound (KD approximately 7 x 10(-11) M) by the Ubx homeodomain peptide; the four central TAAT… CONTINUE READING

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