Optimal 3D single-molecule localization for superresolution microscopy with aberrations and engineered point spread functions

  title={Optimal 3D single-molecule localization for superresolution microscopy with aberrations and engineered point spread functions},
  author={Sean Quirin and Sri Rama Prasanna Pavani and Rafael Piestun},
  journal={Proceedings of the National Academy of Sciences},
  pages={675 - 679}
Photo-activation localization microscopy is a far-field superresolution imaging technique based on the localization of single molecules with subdiffraction limit precision. Known under acronyms such as PALM (photo-activated localization microscopy) or STORM (stochastic optical reconstruction microscopy), these techniques achieve superresolution by allowing only a sparse, random set of molecules to emit light at any given time and subsequently localizing each molecule with great precision… 

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