# Optimal 3D single-molecule localization for superresolution microscopy with aberrations and engineered point spread functions

@article{Quirin2011Optimal3S, title={Optimal 3D single-molecule localization for superresolution microscopy with aberrations and engineered point spread functions}, author={Sean Quirin and Sri Rama Prasanna Pavani and Rafael Piestun}, journal={Proceedings of the National Academy of Sciences}, year={2011}, volume={109}, pages={675 - 679} }

Photo-activation localization microscopy is a far-field superresolution imaging technique based on the localization of single molecules with subdiffraction limit precision. Known under acronyms such as PALM (photo-activated localization microscopy) or STORM (stochastic optical reconstruction microscopy), these techniques achieve superresolution by allowing only a sparse, random set of molecules to emit light at any given time and subsequently localizing each molecule with great precision…

## 119 Citations

### Three dimensional single molecule localization using a phase retrieved pupil function.

- PhysicsOptics express
- 2013

The use of phase-retrieved pupil functions are described to generate a more accurate PSF and therefore more accurate 3D localizations and the superior localization accuracy of the pupil function generated PSF is demonstrated with dual focal plane 3D superresolution imaging of biological structures.

### Super-resolution photon-efficient imaging by nanometric double-helix point spread function localization of emitters (SPINDLE).

- PhysicsOptics express
- 2012

3D super-resolution imaging using photo-switchable dyes reveals the 3D microtubule network in mammalian cells with localization precision approaching the information theoretical limit over a depth of 1.2 µm.

### Improved single-molecule localization precision in astigmatism-based 3D superresolution imaging using weighted likelihood estimation

- Physics
- 2018

This approach numerically approximated the probability of observing a certain signal in a given pixel from a single emitter at a particular z-plane and used a weighted maximum likelihood estimator (WLE) to determine the 3D-position of the emitter.

### Advances in 3D single particle localization microscopy

- BiologyAPL Photonics
- 2019

The principles of operation and typical applications of the most promising 3D localization microscopy techniques are described and a comparison of the attainable precision for each technique in terms of the Cramer-Rao lower bound for high-resolution imaging is provided.

### Three-dimensional biplane spectroscopic single-molecule localization microscopy

- PhysicsOptica
- 2019

Spectroscopic single-molecule localization microscopy (sSMLM) captures the full emission spectra of individual molecules while simultaneously localizing their spatial locations at a precision greatly…

### Addressing systematic errors in axial distance measurements in single-emitter localization microscopy.

- PhysicsOptics express
- 2020

A new experimental system is reported which reproduces a widely-adopted geometry in high-numerical aperture localization microscopy, in which molecules are situated in an aqueous medium above a glass coverslip imaged with an oil-immersion objective.

### Super-resolution image reconstruction for high-density 3D single-molecule microscopy

- Computer Science2016 IEEE 13th International Symposium on Biomedical Imaging (ISBI)
- 2016

This paper proposes a novel super-resolution algorithm for 3D image reconstruction, dubbed TVSTORM, which promotes the sparsity of activated emitters without discretizing their locations, and reduces the computational time by an order-of-magnitude.

### Analysis of super-resolution single molecule localization microscopy data: A tutorial

- ChemistryAIP Advances
- 2022

The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length…

### Simultaneous, accurate measurement of the 3D position and orientation of single molecules

- PhysicsProceedings of the National Academy of Sciences
- 2012

This work exploits parameters uniquely inherent in the double-lobed nature of the Double-Helix Point Spread Function to account for mislocalizations during an axial scan of a single molecule and simultaneously measure 3D molecular orientation and 3D position.

### Optimal 3D single-molecule localization in real time using experimental point spread functions

- PhysicsNature Methods
- 2018

We present a real-time fitter for 3D single-molecule localization microscopy using experimental point spread functions (PSFs) that achieves minimal uncertainty in 3D on any microscope and is…

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