Optically triggering spatiotemporally confined GPCR activity in a cell and programming neurite initiation and extension.

@article{Karunarathne2013OpticallyTS,
  title={Optically triggering spatiotemporally confined GPCR activity in a cell and programming neurite initiation and extension.},
  author={W. K. Ajith Karunarathne and Lopamudra Giri and V. Varun Kalyanaraman and Narasimhan Gautam},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2013},
  volume={110 17},
  pages={E1565-74}
}
G-protein-coupled receptor (GPCR) activity gradients evoke important cell behavior but there is a dearth of methods to induce such asymmetric signaling in a cell. Here we achieved reversible, rapidly switchable patterns of spatiotemporally restricted GPCR activity in a single cell. We recruited properties of nonrhodopsin opsins--rapid deactivation, distinct spectral tuning, and resistance to bleaching--to activate native Gi, Gq, or Gs signaling in selected regions of a cell. Optical inputs were… CONTINUE READING
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