Optical thermophoresis for quantifying the buffer dependence of aptamer binding.

@article{Baaske2010OpticalTF,
  title={Optical thermophoresis for quantifying the buffer dependence of aptamer binding.},
  author={P. Baaske and Christoph J. Wienken and Philipp Reineck and S. Duhr and D. Braun},
  journal={Angewandte Chemie},
  year={2010},
  volume={49 12},
  pages={
          2238-41
        }
}
Quantification of biomolecular binding reactions in theirnative environment is crucial for biology and medicine.However, reliable methods are rare. We have developed anew immobilization-free method in which thermophoresis,the movement of molecules in a thermal gradient, is used todetermine binding curves; this method can be used to studybinding in various buffers as well as in human blood serum.The assay does not rely on surface contact and requires onlyan unspecific fluorescence marker on one… Expand
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