Optical sectioning microscopy

  title={Optical sectioning microscopy},
  author={Jos{\'e}-Angel Conchello and Jeff William Lichtman},
  journal={Nature Methods},
Confocal scanning microscopy, a form of optical sectioning microscopy, has radically transformed optical imaging in biology. These devices provide a powerful means to eliminate from images the background caused by out-of-focus light and scatter. Confocal techniques can also improve the resolution of a light microscope image beyond what is achievable with widefield fluorescence microscopy. The quality of the images obtained, however, depends on the user's familiarity with the optical and… CONTINUE READING
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