Optical probing of sodium dynamics in neurons and astrocytes

  title={Optical probing of sodium dynamics in neurons and astrocytes},
  author={Christophe M. Lamy and Jean-Yves Chatton},
  volume={58 2},
Changes in intracellular Na(+) concentration underlie essential neurobiological processes, but few reliable tools exist for their measurement. Here we characterize a new synthetic Na(+)-sensitive fluorescent dye, Asante Natrium Green (ANG), with unique properties. This indicator was excitable in the visible spectrum and by two-photon illumination, suffered little photobleaching and located to the cytosol were it remained for long durations without noticeable unwanted effects on basic cell… CONTINUE READING
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