Optical principle of pH measurement for detection of auxin flow through cellular membrane

  title={Optical principle of pH measurement for detection of auxin flow through cellular membrane},
  author={Ondřej Podrazk{\'y} and Jan Mr{\'a}zek and Miroslav Seidl and Ivan Kas{\'i}k and Petr Tobi{\vs}ka and Vlastimil Matějec and Tomas Martan and Jan Aubrecht},
  booktitle={SPIE Optics + Optoelectronics},
The paper shows an approach to the determination of pH changes of solutions with a fine spatial resolution by means of fiber-optic tapers and fluorescence detection. This approach can be adopted for the determination of auxin flow through celluar membranes. Spectral absorption and fluorescence of pH transducers, namely of fluorescein, carboxyfluorescein, 6,8-dihydroxy-1,3-pyrenedisulfonic acid disodium salt and 2',7'-bis(2-carbonylethyl)-5(6)-carboxyfluorescein, were tested. The approach, based… Expand
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Conditional gain-of-function alleles and quantitative measurements of auxin accumulation revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors, which suggests a direct involvement ofPINs in catalyzing cellular auxIn efflux. Expand
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Lakowicz, Principles of fluorescence spectroscopy
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