Optical measurements of intracellular pH in single LLC-PK1 cells: demonstration of Cl-HCO3 exchange.

@article{Chaillet1986OpticalMO,
  title={Optical measurements of intracellular pH in single LLC-PK1 cells: demonstration of Cl-HCO3 exchange.},
  author={John Richard Chaillet and Kurt Amsler and Walter F. Boron},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1986},
  volume={83 2},
  pages={522-6}
}
An optical method was used to continuously monitor intracellular pH (pHi) in single cultured LLC-PK1 cells. Rapidly growing or quiescent cells, attached to coverslips, were loaded with the pH-sensitive dye 4',5'-dimethyl-5(and -6)-carboxyfluorescein by exposing them to the dye's permeant precursor. pHi was calculated from the intracellular absorbance spectrum of the dye in a single cell. For cells incubated in HCO3(-)-free Ringer's solution, pHi recovered exponentially from acid loads applied… CONTINUE READING
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