Optical mapping and sequencing of the Escherichia coli KO11 genome reveal extensive chromosomal rearrangements, and multiple tandem copies of the Zymomonas mobilispdc and adhB genes

@article{Turner2011OpticalMA,
  title={Optical mapping and sequencing of the Escherichia coli KO11 genome reveal extensive chromosomal rearrangements, and multiple tandem copies of the Zymomonas mobilispdc and adhB genes},
  author={Peter C. Turner and Lorraine P. Yomano and Laura R. Jarboe and Sean W. York and Christy L. Baggett and Br{\'e}lan E. Moritz and Emily B. Zentz and Keelnatham T Shanmugam and Lonnie O'Neal Ingram},
  journal={Journal of Industrial Microbiology \& Biotechnology},
  year={2011},
  volume={39},
  pages={629-639}
}
Escherichia coli KO11 (ATCC 55124) was engineered in 1990 to produce ethanol by chromosomal insertion of the Zymomonas mobilispdc and adhB genes into E. coli W (ATCC 9637). KO11FL, our current laboratory version of KO11, and its parent E. coli W were sequenced, and contigs assembled into genomic sequences using optical NcoI restriction maps as templates. E. coli W contained plasmids pRK1 (102.5 kb) and pRK2 (5.4 kb), but KO11FL only contained pRK2. KO11FL optical maps made with AflII and with… Expand
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