Optical control of cell signaling by single-chain photoswitchable kinases

  title={Optical control of cell signaling by single-chain photoswitchable kinases},
  author={Xin X Zhou and Linlin Z Fan and Pengpeng Li and Kang Shen and Michael Z. Lin},
Protein kinases transduce signals to regulate a wide array of cellular functions in eukaryotes. A generalizable method for optical control of kinases would enable fine spatiotemporal interrogation or manipulation of these various functions. We report the design and application of single-chain cofactor-free kinases with photoswitchable activity. We engineered a dimeric protein, pdDronpa, that dissociates in cyan light and reassociates in violet light. Attaching two pdDronpa domains at rationally… CONTINUE READING
Recent Discussions
This paper has been referenced on Twitter 74 times over the past 90 days. VIEW TWEETS

From This Paper

Figures, tables, and topics from this paper.


Publications citing this paper.
Showing 1-8 of 8 extracted citations


Publications referenced by this paper.

Fu for assistance with protein structure refinement; J. Philo for assistance with sedimentation equilibrium analysis; L. Tao and G. Colakoglu for assistance with the worm

  • C. Y. Ou
  • Cell 141,
  • 2010
1 Excerpt

Similar Papers

Loading similar papers…