Optical bioimaging: from living tissue to a single molecule: single-molecule visualization of cell signaling processes of epidermal growth factor receptor.

@article{Sako2003OpticalBF,
  title={Optical bioimaging: from living tissue to a single molecule: single-molecule visualization of cell signaling processes of epidermal growth factor receptor.},
  author={Yasushi Sako and Junya Ichinose and Miki Morimatsu and Kosuke Ohta and Takeshi Uyemura},
  journal={Journal of pharmacological sciences},
  year={2003},
  volume={93 3},
  pages={
          253-8
        }
}
Single-molecule imaging is an ideal technology to study molecular mechanisms of biological reactions in vitro. Recently, this technology has been extended to real-time observation of fluorescent dye-labeled molecules in living cells. Total internal reflection fluorescence microscopy is the major technique for this purpose. Using this technique, we have studied the process of early signal transduction of epidermal growth factor (EGF) in single molecules: binding of EGF to its receptor (EGFR) on… CONTINUE READING