Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy

@article{Huisken2004OpticalSD,
  title={Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy},
  author={Jan Huisken and Jim Swoger and Filippo Del Bene and Joachim Wittbrodt and Ernst H. K. Stelzer},
  journal={Science},
  year={2004},
  volume={305},
  pages={1007 - 1009}
}
Large, living biological specimens present challenges to existing optical imaging techniques because of their absorptive and scattering properties. We developed selective plane illumination microscopy (SPIM) to generate multidimensional images of samples up to a few millimeters in size. The system combines two-dimensional illumination with orthogonal camera-based detection to achieve high-resolution, optically sectioned imaging throughout the sample, with minimal photodamage and at speeds… 

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