Optical Imaging of Calcium Transients in Neurons and Pharyngeal Muscle of C. elegans

  title={Optical Imaging of Calcium Transients in Neurons and Pharyngeal Muscle of C. elegans},
  author={Rex Kerr and Varda Lev-Ram and Geoff Baird and Pierre Vincent and Roger Tsien and William R. Schafer},
Electrophysiology and optical indicators have been used in vertebrate systems to investigate excitable cell firing and calcium transients, but both techniques have been difficult to apply in organisms with powerful reverse genetics. To overcome this limitation, we expressed cameleon proteins, genetically encoded calcium indicators, in the pharyngeal muscle of the nematode worm Caenorhabditis elegans. In intact transgenic animals expressing cameleons, fluorescence ratio changes accompanied… CONTINUE READING
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