Opportunities and challenges of the tag-assisted protein purification techniques: Applications in the pharmaceutical industry.

  title={Opportunities and challenges of the tag-assisted protein purification techniques: Applications in the pharmaceutical industry.},
  author={Mohammad M Gomari and N. Saraygord-Afshari and Marziye Farsimadan and Neda Rostami and Shahin Aghamiri and Mohammad Morad Farajollahi},
  journal={Biotechnology advances},

Expression, purification, and kinetic characterization of the human strep-IDO1

Improved method for the purification of recombinant IDO1 protein based on the strep-tag using an E. coli expression system is described, which could be used for further biochemical and structural analyses, which may facilitate functional research and further drug screening study onIDO1.

Optimizing the Preparation Procedure of Recombinant PSCA, as a Practical Biomarker in Prostate Cancer

An analytical study was designed to improve the quality and quantity of PSCA production by introducing a newly designed method to fit the delicate immunological surveys and overcome some limiting factors in P SCA production.

Strategies for the Production of Soluble Interferon-Alpha Consensus and Potential Application in Arboviruses and SARS-CoV-2

It is shown, for the first time, that cIFN has a potent activity against these viruses, being very low amounts of the molecule sufficient to inhibit virus multiplication and this molecule could be used in a clinical approach to treat Arboviruses and SARS-CoV-2.

Engineering His-Tagged Senecavirus A for One-Step Purification of Viral Antigens

The constructed 6×His-tagged SVA may offer a feasible approach to the affinity purification and concentration of antigens in the process of SVA inactivated vaccine production.



Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems

  • K. Terpe
  • Biology
    Applied Microbiology and Biotechnology
  • 2002
An overview of the most frequently used and interesting recombinant hybrids containing a polypeptide fusion partner, termed affinity tag, to facilitate the purification of the targetpolypeptides are given.

New trends in aggregating tags for therapeutic protein purification

This review summarizes the current state-of-the-art in using aggregating tags for protein purification and categorized them into tags that allow soluble expression of target protein in vivo and induce aggregation in vitro.

Several Affinity Tags Commonly Used in Chromatographic Purification

The benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, Strep II tag, streptavidin-binding peptide (SBP) tag, calmodulin-binding proteins (CBP), glutathione S-transferase (GST), maltose-binding protein (MBP), S-tag, HA tag, and c-Myc tag are discussed.

Column-Free Purification Methods for Recombinant Proteins Using Self-Cleaving Aggregating Tags

Two non-chromatographic purification strategies that use either the elastin-like polypeptide (ELP) tag or the β-roll tag in combination with an engineered split intein for tag removal are developed, resulting in increased yields compared to previous ELP and BRT17-based methods.

New trends and affinity tag designs for recombinant protein purification.

  • D. Wood
  • Biology
    Current opinion in structural biology
  • 2014

Polyionic Tags as Enhancers of Protein Solubility in Recombinant Protein Expression

A range of experimentally tested peptide tags, which present specific advantages compared to protein fusion tags and the concluding remarks of these experiments are reviewed and a concept to design solubility-enhancing peptide tagged based on a protein’s pI is suggested.

MAP Tag: A Novel Tagging System for Protein Purification and Detection

A novel affinity tag system composed of a rat anti-mouse podoplanin monoclonal antibody and MAP tag derived from the platelet aggregation-stimulating domain of mouse Podoplanin is developed, indicating that the MAP tag system is suitable for protein purification.