Opiate regulation of IL-1beta and TNF-alpha in cultured human articular chondrocytes.

@article{Andjelkov2005OpiateRO,
  title={Opiate regulation of IL-1beta and TNF-alpha in cultured human articular chondrocytes.},
  author={Nenad Andjelkov and Jan Elvenes and J. -B. Martin and Oddmund Johansen},
  journal={Biochemical and biophysical research communications},
  year={2005},
  volume={333 4},
  pages={1295-9}
}
In order to investigate if beta-endorphins anti-inflammatory effect in cartilage-damaging states is mediated via tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta), we examined its influence on these two cytokines in vitro. Human articular chondrocytes were obtained from patients undergoing total knee arthroplasty and stimulated with beta-endorphin (60-6000 ng/ml). Protein levels of TNF-alpha and IL-1 beta were measured by ELISA in supernatants from articular chondrocyte… CONTINUE READING