Operator sequence alters gene expression independently of transcription factor occupancy in bacteria.

@article{Garcia2012OperatorSA,
  title={Operator sequence alters gene expression independently of transcription factor occupancy in bacteria.},
  author={Hernan G. Garcia and Alvaro Sanchez and James Q. Boedicker and Melisa L. Osborne and Jeff Gelles and Jan{\'e} Kondev and Rob Phillips},
  journal={Cell reports},
  year={2012},
  volume={2 1},
  pages={150-61}
}
A canonical quantitative view of transcriptional regulation holds that the only role of operator sequence is to set the probability of transcription factor binding, with operator occupancy determining the level of gene expression. In this work, we test this idea by characterizing repression in vivo and the binding of RNA polymerase in vitro in experiments where operators of various sequences were placed either upstream or downstream from the promoter in Escherichia coli. Surprisingly, we find… CONTINUE READING
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