Opening of size-selective pores in endosomes during human rhinovirus serotype 2 in vivo uncoating monitored by single-organelle flow analysis.

@article{Brabec2005OpeningOS,
  title={Opening of size-selective pores in endosomes during human rhinovirus serotype 2 in vivo uncoating monitored by single-organelle flow analysis.},
  author={Marianne Brabec and Daniela Schober and Ernst Wagner and Nora Bayer and Robert F. Murphy and D. Blaas and Renate Fuchs},
  journal={Journal of virology},
  year={2005},
  volume={79 2},
  pages={1008-16}
}
The effect of virus uncoating on endosome integrity during the early steps in viral infection was investigated. Using fluid-phase uptake of 10- and 70-kDa dextrans labeled with a pH-dependent fluorophore (fluorescein isothiocyanate [FITC]) and a pH-independent fluorophore (cyanine 5 [Cy5]), we determined the pHs of labeled compartments in intact HeLa cells… CONTINUE READING