Open complex formation by Escherichia coli RNA polymerase: the mechanism of polymerase‐induced strand separation of double helical DNA

@article{Dehaseth1995OpenCF,
  title={Open complex formation by Escherichia coli RNA polymerase: the mechanism of polymerase‐induced strand separation of double helical DNA},
  author={P. Dehaseth and J. Helmann},
  journal={Molecular Microbiology},
  year={1995},
  volume={16}
}
  • P. Dehaseth, J. Helmann
  • Published 1995
  • Biology, Medicine
  • Molecular Microbiology
  • Escherichia coli RNA polymerase is able to site‐specifically melt 12 bp of promoter DNA at temperatures far below those normally associated with DNA melting. Here we consider several models to explain how RNA polymerase destabilizes duplex DNA. One popular model proposes that upon binding to the promoter, RNA polymerase untwists the spacer DNA between the –10 and –35 regions, which results in a destabilization of the –10 region at a TA base step where melting initiates. Promoter untwisting may… CONTINUE READING
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