Ooplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide.

  title={Ooplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide.},
  author={Marcos Roberto Chiaratti and Christina R Ferreira and Felipe Perecin and Simone Cristina M{\'e}o and Juliano Rodrigues Sangalli and L{\'i}gia Garcia Mesquita and J{\'u}lio C{\'e}sar de Carvalho Balieiro and Lawrence Charles Smith and Joaquim Mansano Garcia and Fl{\'a}vio Vieira Meirelles},
  journal={Reproductive biomedicine online},
  volume={22 2},
Ooplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24h during IVM hampered meiotic resumption and the… CONTINUE READING