Ontogeny of T cell receptors in the chicken thymus.

  title={Ontogeny of T cell receptors in the chicken thymus.},
  author={R. Pat Bucy and Chen-lo H. Chen and Max D. Cooper},
  journal={Journal of immunology},
  volume={144 4},
A panel of murine mAb against chicken TCR and associated molecules was used to study the ontogeny of T cells. The intrathymic maturation of the TCR-gamma delta, (TCR-1) and TCR-alpha beta (TCR-2) sublineages was the focus of these studies employing immunoperoxidase staining of tissue sections and immunofluorescence analysis of cell suspensions. The first CD3+ cells appeared in the thymus on embryonic day 9 (E9) when the CD3 Ag was restricted to the cytoplasm. In tissue sections, both TCR-1+ and… CONTINUE READING