Only the last amino acids in the nascent peptide influence translation termination in Escherichia coli genes.

@article{MottaguiTabar1997OnlyTL,
  title={Only the last amino acids in the nascent peptide influence translation termination in Escherichia coli genes.},
  author={Salim Mottagui-Tabar and Leif A. Isaksson},
  journal={FEBS letters},
  year={1997},
  volume={414 1},
  pages={165-70}
}
Efficiency of translation termination is affected if the last two amino acids in the nascent peptide are changed [1,2]. By changing the corresponding codons upstream of the stop signal UGAA, we have analyzed if the -3 to -6 amino acids at the C-terminal region of the nascent peptide also affect termination. Lysine at position -3 gave increased readthrough, whereas a total of 28 variations at positions -4, -5, and -6 showed no significant effect on readthrough. The 3'-ends corresponding to the… CONTINUE READING