Online size-exclusion high-performance liquid chromatography light scattering and differential refractometry methods to determine degree of polymer conjugation to proteins and protein-protein or protein-ligand association states.

@article{Kendrick2001OnlineSH,
  title={Online size-exclusion high-performance liquid chromatography light scattering and differential refractometry methods to determine degree of polymer conjugation to proteins and protein-protein or protein-ligand association states.},
  author={Brent S. Kendrick and Bruce A. Kerwin and B S Chang and John S. Philo},
  journal={Analytical biochemistry},
  year={2001},
  volume={299 2},
  pages={
          136-46
        }
}
Characterizing the solution structure of protein-polymer conjugates and protein-ligand interactions is important in fields such as biotechnology and biochemistry. Size-exclusion high-performance liquid chromatography with online classical light scattering (LS), refractive index (RI), and UV detection offers a powerful tool in such characterization. Novel methods are presented utilizing LS, RI, and UV signals to rapidly determine the degree of conjugation and the molecular mass of the protein… Expand
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Simultaneous determination of protein aggregation, degradation, and absolute molecular weight by size exclusion chromatography-multiangle laser light scattering.
  • Hongping Ye
  • Chemistry, Medicine
  • Analytical biochemistry
  • 2006
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