Online nanoscale ERLIC-MS outperforms RPLC-MS for shotgun proteomics in complex mixtures.

Abstract

We have explored the use of electrostatic repulsion hydrophilic interaction chromatography (ERLIC) as an alternative to the gold-standard in shotgun proteomics: reversed-phase (RP) LC for online ESI-MS/MS. Conditions for sample solubilization and initial gradient conditions were optimized to strike a balance between peptide solubility and maximum peptide… (More)
DOI: 10.1021/pr300638n

Topics

Cite this paper

@article{Jong2012OnlineNE, title={Online nanoscale ERLIC-MS outperforms RPLC-MS for shotgun proteomics in complex mixtures.}, author={Ebbing P. de Jong and Timothy J. Griffin}, journal={Journal of proteome research}, year={2012}, volume={11 10}, pages={5059-64} }